Hotspot

rps16-trnQ-UUG

LSC · 5953-7193 · 1241 bp

Back to Crassula

Pi 0.0601
MarkerSeek score 76.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1241
variable_sites
227
pi
0.0601
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CTAGATCCTTGCCCCTGAGA CTTACAGCAGCTTGCCAAAC 55.0 50.0 57.9 57.9 1758-2044 bp 0.833333 84.1
rps16-trnQ-UUG_p2 CTAGATCCTTGCCCCTGAGA GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1214-1499 bp 0.833333 84.0
rps16-trnQ-UUG_p3 TCCTTGCCCCTGAGAACTAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1294-1494 bp 0.833333 80.1
rps16-trnQ-UUG_p4 TCCTTGCCCCTGAGAACTAA CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1839-2039 bp 0.833333 79.8
rps16-trnQ-UUG_p5 TCCTTGCCCCTGAGAACTAA GTGTTCGCGCACCTATCTAA 50.0 50.0 58.0 58.1 1971-2171 bp 0.666667 72.3