Hotspot

trnK-UUU-rps16

LSC · 4240-4819 · 580 bp

Back to Crassula

Pi 0.0678

MarkerSeek score 77.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 121
pi: 0.0678
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGTCGTGGTCGTACAAAC	TCTATCCCAATGAGCCGTCT	50.0	50.0	57.9	57.9	928-1150 bp	1.0	90.3
trnK-UUU-rps16_p2	CAGTCGTGGTCGTACAAACT	TCTATCCCAATGAGCCGTCT	50.0	50.0	57.9	57.9	927-1149 bp	1.0	90.3
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.2	57.9	842-1064 bp	1.0	89.9
trnK-UUU-rps16_p4	CGGCTTCTTCTACACAACCA	TCTATCCCAATGAGCCGTCT	50.0	50.0	57.8	57.9	1277-1320 bp	0.75	74.5
trnK-UUU-rps16_p5	ACGGCTTCTTCTACACAACC	TCTATCCCAATGAGCCGTCT	50.0	50.0	57.8	57.9	1278-1321 bp	0.75	74.5