Hotspot

trnH-GUG-psbA

LSC · 72-372 · 301 bp

Back to Crassula

Pi 0.087

MarkerSeek score 75.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 87
pi: 0.087
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTGCTGTTGAAGTTCCAT	55.0	45.0	58.1	57.7	339-377 bp	1.0	88.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	353-391 bp	1.0	88.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	352-390 bp	1.0	88.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	354-392 bp	1.0	88.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GCTGCTGTTGAAGTTCCATC	55.0	50.0	58.1	57.4	338-376 bp	1.0	86.0