Hotspot

rps15

SSC · 122700-122966 · 267 bp

Back to Crambe

Pi 0.00375
MarkerSeek score 40.00
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
267
variable_sites
1
pi
0.00375
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 GCTGGCCCTTTTAGGAACTT TCAAATCCTTTCTTTGGCCCA 50.0 42.9 58.1 58.0 1133-1139 bp 1.0 72.8
rps15_p2 TAAGCGAAGAACACCATGCA TCAAATCCTTTCTTTGGCCCA 45.0 42.9 57.8 58.0 920-926 bp 1.0 72.2
rps15_p3 TAATCCCAACGCCTTCAACC TCAAATCCTTTCTTTGGCCCA 50.0 42.9 58.2 58.0 1461-1467 bp 1.0 71.6
rps15_p4 ATCCCACCGCGTTACATAAG TCAAATCCTTTCTTTGGCCCA 50.0 42.9 57.8 58.0 1049-1055 bp 1.0 71.6
rps15_p5 AGCTAAATAATCCCACCGCG TCAAATCCTTTCTTTGGCCCA 50.0 42.9 58.1 58.0 1064 bp 0.5 50.8