Hotspot

psbA

LSC · 283-1344 · 1062 bp

Back to Crambe

Pi 0.00188
MarkerSeek score 46.60
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
2
pi
0.00188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AGGTCTAGACTAACGCTAAAGA ATTTGTGTGCTTGGGAGTCC 40.9 50.0 55.8 58.4 1135 bp 1.0 43.4
psbA_p2 AGGTCTAGACTAACGCTAAAGAA ATTTGTGTGCTTGGGAGTCC 39.1 50.0 56.5 58.4 1135 bp 1.0 41.2
psbA_p3 GGTCTAGACTAACGCTAAAGA ATTTGTGTGCTTGGGAGTCC 42.9 50.0 54.3 58.4 1134 bp 1.0 40.1
psbA_p4 AGGTCTAGACTAACGCTAAAG ATTTGTGTGCTTGGGAGTCC 42.9 50.0 54.3 58.4 1135 bp 1.0 40.1
psbA_p5 AGGTCTAGACTAACGCTAAAGA AGAATTTGTGTGCTTGGGAGT 40.9 42.9 55.8 58.1 1138 bp 1.0 40.1