Hotspot

ccsA-ndhD

SSC · 114854-115067 · 214 bp

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Pi 0.0333
MarkerSeek score 73.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
214
variable_sites
7
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TCTGCAATTGTGGCTTCGAT AGCAAGTGACAAGGTCGAAT 45.0 45.0 58.2 57.4 369-371 bp 1.0 76.6
ccsA-ndhD_p2 TTCTGCAATTGTGGCTTCGA AGCAAGTGACAAGGTCGAAT 45.0 45.0 58.4 57.4 370-372 bp 1.0 74.8
ccsA-ndhD_p3 TCTGCAATTGTGGCTTCGAT GCAAGTGACAAGGTCGAATC 45.0 50.0 58.2 57.1 368-370 bp 1.0 73.9
ccsA-ndhD_p4 ATTCTGCAATTGTGGCTTCG AGCAAGTGACAAGGTCGAAT 45.0 45.0 57.4 57.4 371-373 bp 1.0 72.8
ccsA-ndhD_p5 TTCTGCAATTGTGGCTTCGA GCAAGTGACAAGGTCGAATC 45.0 50.0 58.4 57.1 369-371 bp 1.0 72.1