Hotspot

rpl32-trnL-UAG

SSC · 112987-113673 · 687 bp

Back to Crambe

Pi 0.027
MarkerSeek score 63.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
687
variable_sites
18
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGAGCACTCTCATACGGATT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 56.1 57.9 1093-1100 bp 1.0 66.7
rpl32-trnL-UAG_p2 TGAGCACTCTCATACGGATT TAACCACTGGGACTGGAGAG 45.0 55.0 56.1 58.1 1664-1671 bp 1.0 66.4
rpl32-trnL-UAG_p3 AGGAAGGGATATTGGACATCCT TAACCACTGGGACTGGAGAG 45.5 55.0 58.0 58.1 1380-1393 bp 1.0 66.0
rpl32-trnL-UAG_p4 GAGCACTCTCATACGGATTTCA TAACCACTGGGACTGGAGAG 45.5 55.0 58.0 58.1 1663-1670 bp 1.0 65.5
rpl32-trnL-UAG_p5 TGAGCACTCTCATACGGATTTC TAACCACTGGGACTGGAGAG 45.5 55.0 58.0 58.1 1664-1671 bp 1.0 65.5