Hotspot

psbT-psbN

LSC · 73549-73613 · 65 bp

Back to Crambe

Pi 0.0615
MarkerSeek score 75.10
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
65
variable_sites
4
pi
0.0615
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 GGGAACCGCCTAAAATTTCA GATCCATTCGAGGAACACGG 45.0 55.0 56.3 58.4 124 bp 1.0 65.6
psbT-psbN_p2 GGGAACCGCCTAAAATTTCA AGATCCATTCGAGGAACACG 45.0 50.0 56.3 57.4 125 bp 1.0 64.3
psbT-psbN_p3 GGGAACCGCCTAAAATTTCA GGCAACCCTCTCAACAACTA 45.0 50.0 56.3 57.4 147 bp 1.0 64.1
psbT-psbN_p4 GGGAACCGCCTAAAATTTCAA GATCCATTCGAGGAACACGG 42.9 55.0 57.1 58.4 124 bp 1.0 63.6
psbT-psbN_p5 GGGAACCGCCTAAAATTTCAA GGCAACCCTCTCAACAACTA 42.9 50.0 57.1 57.4 147 bp 1.0 62.1