Hotspot

psaJ-rpl33

LSC · 66274-66704 · 431 bp

Back to Crambe

Pi 0.0307
MarkerSeek score 74.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
431
variable_sites
13
pi
0.0307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GGTTCGTTTCGTTAGCAGGT ATATCTGGAAATTCCCGCGG 50.0 50.0 58.5 57.8 602-607 bp 1.0 77.3
psaJ-rpl33_p2 TGGTTCGTTTCGTTAGCAGG ATATCTGGAAATTCCCGCGG 50.0 50.0 58.5 57.8 603-608 bp 1.0 77.3
psaJ-rpl33_p3 GGTTCGTTTCGTTAGCAGGT AATTCCCGCGGATTCTTTCT 50.0 45.0 58.5 57.6 593-598 bp 1.0 75.5
psaJ-rpl33_p4 GGTTCGTTTCGTTAGCAGGT ATTCCCGCGGATTCTTTCTT 50.0 45.0 58.5 57.6 592-597 bp 1.0 75.5
psaJ-rpl33_p5 TGGTTCGTTTCGTTAGCAGG ATTCCCGCGGATTCTTTCTT 50.0 45.0 58.5 57.6 593-598 bp 1.0 75.5