Hotspot

psbE-petL

LSC · 63584-64938 · 1355 bp

Back to Crambe

Pi 0.0126
MarkerSeek score 57.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1355
variable_sites
17
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 57.9 56.3 1501-1506 bp 1.0 67.0
psbE-petL_p2 AATAACCAGCCCGCAATGAA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1502-1507 bp 1.0 66.3
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1419-1424 bp 1.0 66.3
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1414-1419 bp 1.0 66.0
psbE-petL_p5 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGAA 45.0 42.9 57.9 57.0 1501-1506 bp 1.0 65.0