Hotspot

psaA-ycf3

LSC · 40973-41703 · 731 bp

Back to Crambe

Pi 0.00689
MarkerSeek score 63.40
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
731
variable_sites
5
pi
0.00689
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATTGGTTGACGATCACGAGG 50.0 50.0 57.9 58.0 843-847 bp 1.0 79.9
psaA-ycf3_p2 AGAAATGACCGGGTTTAGCC ATTGGTTGACGATCACGAGG 50.0 50.0 57.9 58.0 860-864 bp 1.0 79.9
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AGCACAGAATTGGTTGACGA 50.0 45.0 57.9 57.7 851-855 bp 1.0 78.0
psaA-ycf3_p4 AGAAATGACCGGGTTTAGCC AGCACAGAATTGGTTGACGA 50.0 45.0 57.9 57.7 868-872 bp 1.0 77.9
psaA-ycf3_p5 AGCCCATTCCTCGAAAGAAG ATTGGTTGACGATCACGAGG 50.0 50.0 57.6 58.0 844-848 bp 1.0 77.6