Hotspot

trnS-GCU-trnG-UCC

LSC · 7707-8420 · 714 bp

Back to Crambe

Pi 0.0319
MarkerSeek score 74.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
714
variable_sites
22
pi
0.0319
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGCTTTAATCCACTCAGCC AATCAAACCGAGAGACCCTT 50.0 45.0 58.3 56.5 818-825 bp 1.0 70.1
trnS-GCU-trnG-UCC_p2 ACGCTTTAATCCACTCAGCC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.3 56.5 817-824 bp 1.0 70.1
trnS-GCU-trnG-UCC_p3 CGCTTTAATCCACTCAGCCA AATCAAACCGAGAGACCCTT 50.0 45.0 58.3 56.5 817-824 bp 1.0 70.1
trnS-GCU-trnG-UCC_p4 CGCTTTAATCCACTCAGCCA ATCAAACCGAGAGACCCTTT 50.0 45.0 58.3 56.5 816-823 bp 1.0 70.1
trnS-GCU-trnG-UCC_p5 AAAGCGGAAGGGAAAGAGAG ATCAAACCGAGAGACCCTTT 50.0 45.0 57.5 56.5 886-893 bp 1.0 68.0