Hotspot

ycf1

SSC · 122917-128106 · 5190 bp

Back to Cousinia

Pi 0.00489

MarkerSeek score 43.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5190
variable_sites: 65
pi: 0.00489
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCCTAATAAAATACGGCCA	CTAGGAAATGCCCATTCCCC	42.9	55.0	57.4	58.0	308 bp	1.0	68.7
ycf1_p2	TGCCCTAATAAAATACGGCCA	GGGGACCGTCTCTAGGAAAT	42.9	55.0	57.4	58.2	319 bp	1.0	66.9
ycf1_p3	TGCCCTAATAAAATACGGCCA	GACCGTCTCTAGGAAATGCC	42.9	55.0	57.4	57.8	316 bp	1.0	66.8
ycf1_p4	TGCCCTAATAAAATACGGCCA	GGACCGTCTCTAGGAAATGC	42.9	55.0	57.4	57.8	317 bp	1.0	66.8
ycf1_p5	TGCCCTAATAAAATACGGCCAA	CTAGGAAATGCCCATTCCCC	40.9	55.0	58.1	58.0	308 bp	1.0	64.5