Hotspot

ndhF

SSC · 108988-111222 · 2235 bp

Back to Cousinia

Pi 0.0019

MarkerSeek score 45.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 12
pi: 0.0019
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGAAATGGATTGGATTGGT	GAGGTAAGATCCGGTGAATCG	35.0	52.4	52.7	58.0	2315 bp	0.875	35.4
ndhF_p2	ATGAAATGGATTGGATTGGT	AGGTAAGATCCGGTGAATCG	35.0	50.0	52.7	56.8	2314 bp	0.875	35.4
ndhF_p3	ATGAAATGGATTGGATTGGT	CGAAAGAGGTAAGATCCGGTG	35.0	52.4	52.7	58.2	2320 bp	0.875	35.4
ndhF_p4	ATGAAATGGATTGGATTGGT	CGAAAGAGGTAAGATCCGGT	35.0	50.0	52.7	56.7	2320 bp	0.875	35.4
ndhF_p5	ATGAAATGGATTGGATTGGT	TCGAAAGAGGTAAGATCCGGT	35.0	47.6	52.7	58.3	2321 bp	0.875	35.4