Hotspot

rps15-ycf1

SSC · 122503-122916 · 414 bp

Back to Cousinia

Pi 0.0069

MarkerSeek score 37.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 7
pi: 0.0069
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	AATAATGGGAGCCGCTTGAG	55.0	50.0	58.0	58.0	630-636 bp	1.0	80.7
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1044-1050 bp	1.0	80.4
rps15-ycf1_p3	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	907-913 bp	1.0	80.1
rps15-ycf1_p4	AATAAGCCAGCAACCGTTGA	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	667-673 bp	1.0	80.1
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	CAATGGACTGGACGTGTGAA	50.0	50.0	58.0	58.1	1704-1710 bp	1.0	79.8