Hotspot

trnC-GCA-trnS-GCU

LSC · 74445-75257 · 813 bp

Back to Cousinia

Pi 0.00295

MarkerSeek score 52.10

Primer available yes

Label trnC-GCA-trnS-GCU

Feature Metadata

label_name: trnC-GCA-trnS-GCU
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 7
pi: 0.00295
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnS-GCU_p1	TCAGAGATTCTCGCGAGTCT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	971-1015 bp	1.0	82.9
trnC-GCA-trnS-GCU_p2	CCGGATTTGAACTGGGGAAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	920-964 bp	1.0	82.7
trnC-GCA-trnS-GCU_p3	CACTCAGAGATTCTCGCGAG	ACGGAAAGAGAGGGATTCGA	55.0	50.0	57.9	58.2	974-1018 bp	1.0	82.3
trnC-GCA-trnS-GCU_p4	ACTCAGAGATTCTCGCGAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	973-1017 bp	1.0	81.1
trnC-GCA-trnS-GCU_p5	AACACTCAGAGATTCTCGCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	976-1020 bp	1.0	80.9