Hotspot

psbM-petN

LSC · 73268-73746 · 479 bp

Back to Cousinia

Pi 0.00283

MarkerSeek score 53.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 4
pi: 0.00283
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	55.6	58.2	630-641 bp	1.0	61.3
psbM-petN_p2	TGCTACTGCACTGTTCATTTT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	56.4	58.2	630-641 bp	1.0	59.6
psbM-petN_p3	TGCTACTGCACTGTTCATTT	ATAGTAAGTCTCGCTTGGGC	40.0	50.0	55.6	57.1	631-642 bp	1.0	55.4
psbM-petN_p4	TGCTACTGCACTGTTCATTT	CTCGCTTGGGCTGCTTTAAT	40.0	50.0	55.6	58.9	622-633 bp	1.0	55.3
psbM-petN_p5	TGCTACTGCACTGTTCATTT	TGGGCTGCTTTAATGGTAGTC	40.0	47.6	55.6	58.0	616-627 bp	1.0	54.5