Hotspot

rpoB-trnE-UUC

LSC · 71322-72126 · 805 bp

Back to Cousinia

Pi 0.00825

MarkerSeek score 50.90

Primer available yes

Label rpoB-trnE-UUC

Feature Metadata

label_name: rpoB-trnE-UUC
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 16
pi: 0.00825
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnE-UUC_p1	CATTGCGTCTATCCCGGATT	GCATGATTTACCCCCAGAGG	50.0	55.0	57.8	58.0	940 bp	1.0	79.9
rpoB-trnE-UUC_p2	CATTGCGTCTATCCCGGATT	GGCATGATTTACCCCCAGAG	50.0	55.0	57.8	58.0	941 bp	1.0	79.9
rpoB-trnE-UUC_p3	TGTGGACATTGCGTCTATCC	GCATGATTTACCCCCAGAGG	50.0	55.0	57.7	58.0	946 bp	1.0	78.8
rpoB-trnE-UUC_p4	TGTGGACATTGCGTCTATCC	GGCATGATTTACCCCCAGAG	50.0	55.0	57.7	58.0	947 bp	1.0	78.8
rpoB-trnE-UUC_p5	CATTGCGTCTATCCCGGATT	ACCCTCGTTATCTCCGTGAA	50.0	50.0	57.8	58.2	913 bp	1.0	78.8