Hotspot

psbZ-trnS-UGA

LSC · 48745-49081 · 337 bp

Back to Cousinia

Pi 0.00127

MarkerSeek score 35.50

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CCAACCATCAGGAGAAGCAA	CAACCACTCAGCCATCTCTC	50.0	55.0	57.8	58.0	457 bp	1.0	78.4
psbZ-trnS-UGA_p2	GACCAACCATCAGGAGAAGC	CAACCACTCAGCCATCTCTC	55.0	55.0	58.3	58.0	459 bp	1.0	78.0
psbZ-trnS-UGA_p3	CCAACCATCAGGAGAAGCAA	TCAACCACTCAGCCATCTCT	50.0	50.0	57.8	58.4	458 bp	1.0	75.7
psbZ-trnS-UGA_p4	GACCAACCATCAGGAGAAGC	TCAACCACTCAGCCATCTCT	55.0	50.0	58.3	58.4	460 bp	1.0	75.3
psbZ-trnS-UGA_p5	CCAACCATCAGGAGAAGCAA	TTCAACCACTCAGCCATCTC	50.0	50.0	57.8	57.5	459 bp	1.0	74.7