Hotspot

cemA-pafII

LSC · 22932-23773 · 842 bp

Back to Cousinia

Pi 0.00433

MarkerSeek score 61.10

Primer available yes

Label cemA-pafII

Feature Metadata

label_name: cemA-pafII
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 8
pi: 0.00433
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-pafII_p1	GAGAAATCCACCAGGGCAAA	GAGCCATTCCATTGACTCGT	50.0	50.0	58.1	58.0	989-1029 bp	1.0	82.6
cemA-pafII_p2	GAGAAATCCACCAGGGCAAA	TTGGCCTATTTCTTGCGTGT	50.0	45.0	58.1	58.1	921-961 bp	1.0	82.3
cemA-pafII_p3	GAGAAATCCACCAGGGCAAA	CTCCACGGGAAATGGAACAA	50.0	50.0	58.1	58.1	953-993 bp	1.0	82.2
cemA-pafII_p4	GAGAAATCCACCAGGGCAAA	AAAAAGCTGCTGAATTGGCC	50.0	45.0	58.1	57.8	935-975 bp	1.0	81.7
cemA-pafII_p5	GAGAAATCCACCAGGGCAAA	AAAGCTGCTGAATTGGCCTA	50.0	45.0	58.1	57.8	933-973 bp	1.0	81.3