Hotspot

ycf1

SSC · 112221-117554 · 5334 bp

Back to Coursetia

Pi 0.418

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5334
variable_sites: 1975
pi: 0.418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTAAAAGCAGCATACGCGA	CTAGACAGGCGAACAGGTTC	45.0	55.0	58.0	58.0	423 bp	0.5	59.9
ycf1_p2	CAGTAAAAGCAGCATACGCG	CTAGACAGGCGAACAGGTTC	50.0	55.0	57.9	58.0	424 bp	0.5	59.4
ycf1_p3	AGTAAAAGCAGCATACGCGA	TTACCGAATTTTCAAGCGCG	45.0	45.0	58.0	57.8	928 bp	0.5	58.1
ycf1_p4	CAGTAAAAGCAGCATACGCG	TTACCGAATTTTCAAGCGCG	50.0	45.0	57.9	57.8	929 bp	0.5	57.5
ycf1_p5	GTAAAAGCAGCATACGCGAG	CTAGACAGGCGAACAGGTTC	50.0	55.0	57.6	58.0	422 bp	0.5	57.2