Hotspot

psaC

SSC · 124219-124464 · 246 bp

Back to Coursetia

Pi 0.482

MarkerSeek score 72.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 94
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGAAGCAGCTATCGGATTGG	GAACTTCCCGCAGTGTACAA	50.0	50.0	57.8	58.1	1273 bp	0.5	57.0
psaC_p2	TGAAGCAGCTATCGGATTGG	GGTATTCTCGAGTAACCGGC	50.0	55.0	57.8	57.9	1083 bp	0.5	57.0
psaC_p3	TAGCATGCAGGCTACACAAC	GAACTTCCCGCAGTGTACAA	50.0	50.0	58.3	58.1	1117 bp	0.5	56.9
psaC_p4	TAGCATGCAGGCTACACAAC	GGTATTCTCGAGTAACCGGC	50.0	55.0	58.3	57.9	927 bp	0.5	56.9
psaC_p5	TGAAGCAGCTATCGGATTGG	ATTAAGGAACCCCCGGCTAT	50.0	50.0	57.8	58.2	811 bp	0.5	56.7