Hotspot

ndhI

SSC · 121916-122401 · 486 bp

Back to Coursetia

Pi 0.467

MarkerSeek score 77.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 188
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGTGGGGATGGAATTTGTGG	CAAGAACCAGTCCCAACGAA	50.0	50.0	58.1	58.0	1648 bp	0.5	59.2
ndhI_p2	GAGATAGAAAATCGGCCCGG	CAAGAACCAGTCCCAACGAA	55.0	50.0	58.2	58.0	1825 bp	0.5	58.1
ndhI_p3	CTTTCCAGGAGCCGTATGAG	CAAGAACCAGTCCCAACGAA	55.0	50.0	57.8	58.0	1786 bp	0.5	57.8
ndhI_p4	GGCCCGGTCCTTAGATTTAC	CAAGAACCAGTCCCAACGAA	55.0	50.0	57.7	58.0	1812 bp	0.5	57.7
ndhI_p5	TGTGGGGATGGAATTTGTGG	AACAAGAACCAGTCCCAACG	50.0	50.0	58.1	58.3	1650 bp	0.5	56.9