Hotspot

ndhF

SSC · 128954-131197 · 2244 bp

Back to Coursetia

Pi 0.449

MarkerSeek score 80.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 881
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGCCCATTTTAACTTTGTGC	CACCACATTCTCCATAGGGC	42.9	55.0	57.9	58.0	2807 bp	0.5	50.8
ndhF_p2	TGGCCCATTTTAACTTTGTGC	GCTCGGGTTATGGAAGAAGG	42.9	55.0	57.9	58.0	2764 bp	0.5	50.7
ndhF_p3	TGGCCCATTTTAACTTTGTGC	ATGGAAGAAGGGACCGAGAA	42.9	50.0	57.9	58.0	2755 bp	0.5	50.7
ndhF_p4	TGGCCCATTTTAACTTTGTGC	AGGGCCCTCTTATCTCTTCC	42.9	55.0	57.9	57.9	2792 bp	0.5	50.2
ndhF_p5	TGGCCCATTTTAACTTTGTGC	GGGCCCTCTTATCTCTTCCT	42.9	55.0	57.9	57.9	2791 bp	0.5	50.2