Hotspot

ndhE

SSC · 123624-123929 · 306 bp

Back to Coursetia

Pi 0.43

MarkerSeek score 76.90

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 122
pi: 0.43
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTCGTTGGGACTGGTTCTTG	ACATCCGTCCCAAGGTATCA	50.0	50.0	58.0	58.1	1342 bp	0.5	58.7
ndhE_p2	TTCGTTGGGACTGGTTCTTG	ATTAAGGAACCCCCGGCTAT	50.0	50.0	58.0	58.2	1671 bp	0.5	58.3
ndhE_p3	TTCGTTGGGACTGGTTCTTG	CAAGCGGATTCACACCTCTT	50.0	50.0	58.0	58.2	1413 bp	0.5	58.2
ndhE_p4	TTCGTTGGGACTGGTTCTTG	CCCGGCTATTGGCAAAACTA	50.0	50.0	58.0	58.2	1660 bp	0.5	57.8
ndhE_p5	TTCGTTGGGACTGGTTCTTG	CGGAGGCTATTTGCTTAGCT	50.0	50.0	58.0	57.8	1364 bp	0.5	57.7