Hotspot

ndhA

SSC · 119519-121835 · 2317 bp

Back to Coursetia

Pi 0.467

MarkerSeek score 78.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2317
variable_sites: 912
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCGCCCGGTTTTATCAATT	TTGACCGATGTACCTTGCAG	45.0	50.0	57.9	57.9	2588 bp	0.5	58.2
ndhA_p2	TCCGCCCGGTTTTATCAATT	CGGCCCAAAGCAATTTGATT	45.0	45.0	57.9	57.9	2947 bp	0.5	58.2
ndhA_p3	GCTTCCGGAATACAATGGGA	TTGACCGATGTACCTTGCAG	50.0	50.0	57.7	57.9	2977 bp	0.5	56.5
ndhA_p4	TCCGCCCGGTTTTATCAATT	AAACGGCCCAAAGCAATTTG	45.0	45.0	57.9	58.4	2950 bp	0.5	55.7
ndhA_p5	TCCGCCCGGTTTTATCAATT	GCTCGGACTGTTTGTTGACT	45.0	50.0	57.9	58.4	2569 bp	0.5	55.6