Hotspot

rpl32-ndhF

SSC · 128243-128953 · 711 bp

Back to Coursetia

Pi 0.407

MarkerSeek score 78.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 711
variable_sites: 225
pi: 0.407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCCCCGTAAGAATCGATTGC	TGTTGCCGTGGGAAAAAGTA	50.0	45.0	57.8	57.9	934 bp	0.5	57.7
rpl32-ndhF_p2	TCCCCGTAAGAATCGATTGC	AGGAACAGGAAGTGGAGTGA	50.0	50.0	57.8	57.9	892 bp	0.5	57.3
rpl32-ndhF_p3	TCCCCGTAAGAATCGATTGC	TTTGCTGCAATAGGACGTGT	50.0	45.0	57.8	58.1	1353 bp	0.5	57.3
rpl32-ndhF_p4	TCCCCGTAAGAATCGATTGC	GCAATAGGACGTGTGAACCA	50.0	50.0	57.8	58.2	1347 bp	0.5	56.6
rpl32-ndhF_p5	TCCCCGTAAGAATCGATTGC	TGCAATAGGACGTGTGAACC	50.0	50.0	57.8	58.2	1348 bp	0.5	56.6