Hotspot

ndhI-ndhG

SSC · 122402-122868 · 467 bp

Back to Coursetia

Pi 0.458

MarkerSeek score 78.00

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 467
variable_sites: 181
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CTGCAAGGTACATCGGTCAA	CAAGAACCAGTCCCAACGAA	50.0	50.0	57.9	58.0	1031 bp	0.5	58.9
ndhI-ndhG_p2	AATCAAATTGCTTTGGGCCG	CAAGAACCAGTCCCAACGAA	45.0	50.0	57.9	58.0	672 bp	0.5	58.9
ndhI-ndhG_p3	CTGCAAGGTACATCGGTCAA	CCGACGGTCCAAAGATGAAA	50.0	50.0	57.9	58.2	1188 bp	0.5	57.7
ndhI-ndhG_p4	AATCAAATTGCTTTGGGCCG	CCGACGGTCCAAAGATGAAA	45.0	50.0	57.9	58.2	829 bp	0.5	57.6
ndhI-ndhG_p5	CTGCAAGGTACATCGGTCAA	TTGCACCTATCAAAGCAGCT	50.0	45.0	57.9	57.8	1412 bp	0.5	57.6