Hotspot

trnY-GUA-trnE-UUC

LSC · 40380-40439 · 60 bp

Back to Coursetia

Pi 0.455

MarkerSeek score 77.50

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 25
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	ATCAGCAAATTTTTGGGCCG	ACATCTACCCACCCATTCCA	45.0	50.0	57.9	58.0	751 bp	0.5	59.1
trnY-GUA-trnE-UUC_p2	CCGTCATGTTGGGAGAACAA	ACATCTACCCACCCATTCCA	50.0	50.0	58.1	58.0	818 bp	0.5	59.0
trnY-GUA-trnE-UUC_p3	GTATTCATCGGGACTGACGG	ACATCTACCCACCCATTCCA	55.0	50.0	57.9	58.0	1288 bp	0.5	58.8
trnY-GUA-trnE-UUC_p4	ATCAGCAAATTTTTGGGCCG	AAGTATGCCCCCATCGTCTA	45.0	50.0	57.9	57.9	236 bp	0.5	58.4
trnY-GUA-trnE-UUC_p5	CCGTCATGTTGGGAGAACAA	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	303 bp	0.5	58.4