Hotspot

trnD-GUC-trnY-GUA

LSC · 39828-40295 · 468 bp

Back to Coursetia

Pi 0.438

MarkerSeek score 77.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 168
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GTATTCATCGGGACTGACGG	ACATCTACCCACCCATTCCA	55.0	50.0	57.9	58.0	1288 bp	0.5	58.8
trnD-GUC-trnY-GUA_p2	GTATTCATCGGGACTGACGG	AAGTATGCCCCCATCGTCTA	55.0	50.0	57.9	57.9	773 bp	0.5	58.1
trnD-GUC-trnY-GUA_p3	GTATTCATCGGGACTGACGG	ATGGATTCTTCCTGGGTCGA	55.0	50.0	57.9	58.1	647 bp	0.5	58.0
trnD-GUC-trnY-GUA_p4	GTATTCATCGGGACTGACGG	TGGATTCTTCCTGGGTCGAT	55.0	50.0	57.9	58.1	646 bp	0.5	58.0
trnD-GUC-trnY-GUA_p5	GTATTCATCGGGACTGACGG	TCTCTTTCAAGGAGGCAACG	55.0	50.0	57.9	57.8	738 bp	0.5	57.6