primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnD-GUC_p1 trnD-GUC 1 TCTTCGTCGGAACCTTTACC AAGTATGCCCCCATCGTCTA 20 20 50.0 50.0 57.3 57.9 0.0 0.0 0.827660 39754 39827 898-898 0.500000 53.4 trnD-GUC_p2 trnD-GUC 2 TCTTCGTCGGAACCTTTACC CGGCCCAAAAATTTGCTGAT 20 20 50.0 45.0 57.3 57.9 0.0 0.0 0.828229 39754 39827 682-682 0.500000 53.4 trnD-GUC_p3 trnD-GUC 3 CTTCGTCGGAACCTTTACCT AAGTATGCCCCCATCGTCTA 20 20 50.0 50.0 57.3 57.9 0.0 0.0 0.835123 39754 39827 897-897 0.500000 53.3 trnD-GUC_p4 trnD-GUC 4 CTTCGTCGGAACCTTTACCT CGGCCCAAAAATTTGCTGAT 20 20 50.0 45.0 57.3 57.9 0.0 0.0 0.835693 39754 39827 681-681 0.500000 53.3 trnD-GUC_p5 trnD-GUC 5 TCTTCGTCGGAACCTTTACC TTGTTCTCCCAACATGACGG 20 20 50.0 50.0 57.3 58.1 0.0 0.0 0.839673 39754 39827 615-615 0.500000 53.3 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 GTATTCATCGGGACTGACGG ACATCTACCCACCCATTCCA 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.155855 39828 40295 1288-1288 0.500000 58.8 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 GTATTCATCGGGACTGACGG AAGTATGCCCCCATCGTCTA 20 20 55.0 50.0 57.9 57.9 0.0 0.0 0.232774 39828 40295 773-773 0.500000 58.1 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 GTATTCATCGGGACTGACGG ATGGATTCTTCCTGGGTCGA 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.256090 39828 40295 647-647 0.500000 58.0 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 GTATTCATCGGGACTGACGG TGGATTCTTCCTGGGTCGAT 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.256090 39828 40295 646-646 0.500000 58.0 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 GTATTCATCGGGACTGACGG TCTCTTTCAAGGAGGCAACG 20 20 55.0 50.0 57.9 57.8 0.0 0.0 0.305497 39828 40295 738-738 0.500000 57.6 trnY-GUA-trnE-UUC_p1 trnY-GUA-trnE-UUC 1 ATCAGCAAATTTTTGGGCCG ACATCTACCCACCCATTCCA 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.117870 40380 40439 751-751 0.500000 59.1 trnY-GUA-trnE-UUC_p2 trnY-GUA-trnE-UUC 2 CCGTCATGTTGGGAGAACAA ACATCTACCCACCCATTCCA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.129314 40380 40439 818-818 0.500000 59.0 trnY-GUA-trnE-UUC_p3 trnY-GUA-trnE-UUC 3 GTATTCATCGGGACTGACGG ACATCTACCCACCCATTCCA 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.155855 40380 40439 1288-1288 0.500000 58.8 trnY-GUA-trnE-UUC_p4 trnY-GUA-trnE-UUC 4 ATCAGCAAATTTTTGGGCCG AAGTATGCCCCCATCGTCTA 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.194790 40380 40439 236-236 0.500000 58.4 trnY-GUA-trnE-UUC_p5 trnY-GUA-trnE-UUC 5 CCGTCATGTTGGGAGAACAA AAGTATGCCCCCATCGTCTA 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.206234 40380 40439 303-303 0.500000 58.4 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 ATCAGCAAATTTTTGGGCCG GAACCGATGACTTACGCCTT 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.103650 40513 41316 1089-1089 0.500000 59.2 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 ATCAGCAAATTTTTGGGCCG CATCGGAAATATCGGCGGAT 20 20 45.0 50.0 57.9 58.0 0.0 7.2 0.107825 40513 41316 1566-1566 0.500000 59.1 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 CCGTCATGTTGGGAGAACAA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.115094 40513 41316 1156-1156 0.500000 59.1 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 CCGTCATGTTGGGAGAACAA CATCGGAAATATCGGCGGAT 20 20 50.0 50.0 58.1 58.0 0.0 7.2 0.119269 40513 41316 1633-1633 0.500000 59.0 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 ATCAGCAAATTTTTGGGCCG CCATGGCGTTACTCTACCAC 20 20 45.0 55.0 57.9 58.1 0.0 0.0 0.161747 40513 41316 1068-1068 0.500000 58.7 ycf1_p1 ycf1 1 AGTAAAAGCAGCATACGCGA CTAGACAGGCGAACAGGTTC 20 20 45.0 55.0 58.0 58.0 15.9 2.1 0.006913 116903 117022 423-423 0.500000 59.9 ycf1_p2 ycf1 2 CAGTAAAAGCAGCATACGCG CTAGACAGGCGAACAGGTTC 20 20 50.0 55.0 57.9 58.0 3.5 2.1 0.079486 116903 117022 424-424 0.500000 59.4 ycf1_p3 ycf1 3 AGTAAAAGCAGCATACGCGA TTACCGAATTTTCAAGCGCG 20 20 45.0 45.0 58.0 57.8 15.9 0.0 0.239821 116903 117022 928-928 0.500000 58.1 ycf1_p4 ycf1 4 CAGTAAAAGCAGCATACGCG TTACCGAATTTTCAAGCGCG 20 20 50.0 45.0 57.9 57.8 3.5 0.0 0.312393 116903 117022 929-929 0.500000 57.5 ycf1_p5 ycf1 5 GTAAAAGCAGCATACGCGAG CTAGACAGGCGAACAGGTTC 20 20 50.0 55.0 57.6 58.0 15.9 2.1 0.355588 116903 117022 422-422 0.500000 57.2 ndhH_p1 ndhH 1 AAAAAGGAGGGGGAGAGGAA CCGTCTGCTAGAGCTTGAAG 20 20 50.0 55.0 57.9 58.1 0.0 2.7 0.229603 118336 119517 1836-1836 0.500000 58.2 ndhH_p2 ndhH 2 AAAAGGAGGGGGAGAGGAAA CCGTCTGCTAGAGCTTGAAG 20 20 50.0 55.0 57.9 58.1 0.0 2.7 0.229603 118336 119517 1835-1835 0.500000 58.2 ndhH_p3 ndhH 3 AAAAAGGAGGGGGAGAGGAA TTTGTTCCGTCTGCTAGAGC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.229807 118336 119517 1842-1842 0.500000 58.2 ndhH_p4 ndhH 4 AAAAGGAGGGGGAGAGGAAA TTTGTTCCGTCTGCTAGAGC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.229807 118336 119517 1841-1841 0.500000 58.2 ndhH_p5 ndhH 5 AAAAGGAGGGGGAGAGGAAA TCAGGTCCAATACGCTGTTG 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.242828 118336 119517 1793-1793 0.500000 58.1 ndhA_p1 ndhA 1 TCCGCCCGGTTTTATCAATT TTGACCGATGTACCTTGCAG 20 20 45.0 50.0 57.9 57.9 6.6 0.0 0.225082 119519 121835 2588-2588 0.500000 58.2 ndhA_p2 ndhA 2 TCCGCCCGGTTTTATCAATT CGGCCCAAAGCAATTTGATT 20 20 45.0 45.0 57.9 57.9 6.6 0.0 0.229456 119519 121835 2947-2947 0.500000 58.2 ndhA_p3 ndhA 3 GCTTCCGGAATACAATGGGA TTGACCGATGTACCTTGCAG 20 20 50.0 50.0 57.7 57.9 19.2 0.0 0.435153 119519 121835 2977-2977 0.500000 56.5 ndhA_p4 ndhA 4 TCCGCCCGGTTTTATCAATT AAACGGCCCAAAGCAATTTG 20 20 45.0 45.0 57.9 58.4 6.6 0.0 0.539712 119519 121835 2950-2950 0.500000 55.7 ndhA_p5 ndhA 5 TCCGCCCGGTTTTATCAATT GCTCGGACTGTTTGTTGACT 20 20 45.0 50.0 57.9 58.4 6.6 0.0 0.548722 119519 121835 2569-2569 0.500000 55.6 ndhI_p1 ndhI 1 TGTGGGGATGGAATTTGTGG CAAGAACCAGTCCCAACGAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.102407 121916 122401 1648-1648 0.500000 59.2 ndhI_p2 ndhI 2 GAGATAGAAAATCGGCCCGG CAAGAACCAGTCCCAACGAA 20 20 55.0 50.0 58.2 58.0 1.9 0.0 0.241182 121916 122401 1825-1825 0.500000 58.1 ndhI_p3 ndhI 3 CTTTCCAGGAGCCGTATGAG CAAGAACCAGTCCCAACGAA 20 20 55.0 50.0 57.8 58.0 0.0 0.0 0.269421 121916 122401 1786-1786 0.500000 57.8 ndhI_p4 ndhI 4 GGCCCGGTCCTTAGATTTAC CAAGAACCAGTCCCAACGAA 20 20 55.0 50.0 57.7 58.0 0.0 0.0 0.289615 121916 122401 1812-1812 0.500000 57.7 ndhI_p5 ndhI 5 TGTGGGGATGGAATTTGTGG AACAAGAACCAGTCCCAACG 20 20 50.0 50.0 58.1 58.3 0.0 0.0 0.388649 121916 122401 1650-1650 0.500000 56.9 ndhI-ndhG_p1 ndhI-ndhG 1 CTGCAAGGTACATCGGTCAA CAAGAACCAGTCCCAACGAA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.131513 122402 122868 1031-1031 0.500000 58.9 ndhI-ndhG_p2 ndhI-ndhG 2 AATCAAATTGCTTTGGGCCG CAAGAACCAGTCCCAACGAA 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.135886 122402 122868 672-672 0.500000 58.9 ndhI-ndhG_p3 ndhI-ndhG 3 CTGCAAGGTACATCGGTCAA CCGACGGTCCAAAGATGAAA 20 20 50.0 50.0 57.9 58.2 0.0 5.5 0.289972 122402 122868 1188-1188 0.500000 57.7 ndhI-ndhG_p4 ndhI-ndhG 4 AATCAAATTGCTTTGGGCCG CCGACGGTCCAAAGATGAAA 20 20 45.0 50.0 57.9 58.2 0.0 5.5 0.294346 122402 122868 829-829 0.500000 57.6 ndhI-ndhG_p5 ndhI-ndhG 5 CTGCAAGGTACATCGGTCAA TTGCACCTATCAAAGCAGCT 20 20 50.0 45.0 57.9 57.8 0.0 0.0 0.298329 122402 122868 1412-1412 0.500000 57.6 ndhG_p1 ndhG 1 AATCAAATTGCTTTGGGCCG CCAATCCGATAGCTGCTTCA 20 20 45.0 50.0 57.9 57.8 0.0 7.8 0.342839 122869 123399 1532-1532 0.500000 57.3 ndhG_p2 ndhG 2 AATCAAATTGCTTTGGGCCG GTTGTGTAGCCTGCATGCTA 20 20 45.0 50.0 57.9 58.3 0.0 18.4 0.359841 122869 123399 1688-1688 0.500000 57.1 ndhG_p3 ndhG 3 AATCAAATTGCTTTGGGCCG ATAGCCAATCCGATAGCTGC 20 20 45.0 50.0 57.9 57.6 0.0 13.6 0.490951 122869 123399 1536-1536 0.500000 56.1 ndhG_p4 ndhG 4 AATCAAATTGCTTTGGGCCG TGTTCGAGCATCATTGACCA 20 20 45.0 45.0 57.9 57.5 0.0 0.0 0.568663 122869 123399 1311-1311 0.500000 55.5 ndhG_p5 ndhG 5 AATCAAATTGCTTTGGGCCG TTGTGTAGCCTGCATGCTAA 20 20 45.0 45.0 57.9 57.5 0.0 23.0 0.585884 122869 123399 1687-1687 0.500000 55.3 ndhE_p1 ndhE 1 TTCGTTGGGACTGGTTCTTG ACATCCGTCCCAAGGTATCA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.164077 123624 123929 1342-1342 0.500000 58.7 ndhE_p2 ndhE 2 TTCGTTGGGACTGGTTCTTG ATTAAGGAACCCCCGGCTAT 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.209069 123624 123929 1671-1671 0.500000 58.3 ndhE_p3 ndhE 3 TTCGTTGGGACTGGTTCTTG CAAGCGGATTCACACCTCTT 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.224693 123624 123929 1413-1413 0.500000 58.2 ndhE_p4 ndhE 4 TTCGTTGGGACTGGTTCTTG CCCGGCTATTGGCAAAACTA 20 20 50.0 50.0 58.0 58.2 0.0 3.0 0.280524 123624 123929 1660-1660 0.500000 57.8 ndhE_p5 ndhE 5 TTCGTTGGGACTGGTTCTTG CGGAGGCTATTTGCTTAGCT 20 20 50.0 50.0 58.0 57.8 0.0 5.6 0.284925 123624 123929 1364-1364 0.500000 57.7 psaC_p1 psaC 1 TGAAGCAGCTATCGGATTGG GAACTTCCCGCAGTGTACAA 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.371462 124219 124464 1273-1273 0.500000 57.0 psaC_p2 psaC 2 TGAAGCAGCTATCGGATTGG GGTATTCTCGAGTAACCGGC 20 20 50.0 55.0 57.8 57.9 0.0 13.1 0.376440 124219 124464 1083-1083 0.500000 57.0 psaC_p3 psaC 3 TAGCATGCAGGCTACACAAC GAACTTCCCGCAGTGTACAA 20 20 50.0 50.0 58.3 58.1 27.9 0.0 0.388464 124219 124464 1117-1117 0.500000 56.9 psaC_p4 psaC 4 TAGCATGCAGGCTACACAAC GGTATTCTCGAGTAACCGGC 20 20 50.0 55.0 58.3 57.9 27.9 13.1 0.393442 124219 124464 927-927 0.500000 56.9 psaC_p5 psaC 5 TGAAGCAGCTATCGGATTGG ATTAAGGAACCCCCGGCTAT 20 20 50.0 50.0 57.8 58.2 0.0 0.0 0.416022 124219 124464 811-811 0.500000 56.7 ndhD_p1 ndhD 1 TGATACCTTGGGACGGATGT TCAGGAGCAGTATGGGCTAA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.300556 124585 126081 2268-2268 0.500000 57.6 ndhD_p2 ndhD 2 TGATACCTTGGGACGGATGT TTCAGGAGCAGTATGGGCTA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.300556 124585 126081 2269-2269 0.500000 57.6 ndhD_p3 ndhD 3 TGATACCTTGGGACGGATGT GAGGCGTGGGGATCATATTG 20 20 50.0 55.0 58.1 58.2 0.0 0.0 0.312357 124585 126081 2247-2247 0.500000 57.5 ndhD_p4 ndhD 4 AAGAGGTGTGAATCCGCTTG TCAGGAGCAGTATGGGCTAA 20 20 50.0 50.0 58.2 57.8 0.6 0.0 0.361172 124585 126081 2197-2197 0.500000 57.1 ndhD_p5 ndhD 5 AAGAGGTGTGAATCCGCTTG TTCAGGAGCAGTATGGGCTA 20 20 50.0 50.0 58.2 57.8 0.6 0.0 0.361172 124585 126081 2198-2198 0.500000 57.1 trnL-UAG-rpl32_p1 trnL-UAG-rpl32 1 GAAGTAGCAAAGCCTTGGGT GCAATCGATTCTTACGGGGA 20 20 50.0 50.0 58.1 57.8 0.0 7.9 0.323242 127495 128086 1182-1182 0.500000 57.4 trnL-UAG-rpl32_p2 trnL-UAG-rpl32 2 ATGGGATTGTAGAGCGGGTA GCAATCGATTCTTACGGGGA 20 20 50.0 50.0 57.9 57.8 0.0 7.9 0.331410 127495 128086 1240-1240 0.500000 57.3 trnL-UAG-rpl32_p3 trnL-UAG-rpl32 3 GATAAGATGTCATGCCGCCA GCAATCGATTCTTACGGGGA 20 20 50.0 50.0 58.1 57.8 0.0 7.9 0.351387 127495 128086 736-736 0.500000 57.2 trnL-UAG-rpl32_p4 trnL-UAG-rpl32 4 TCCTAAGAGCAGCGTGTCTA GCAATCGATTCTTACGGGGA 20 20 50.0 50.0 57.9 57.8 0.0 7.9 0.358577 127495 128086 683-683 0.500000 57.1 trnL-UAG-rpl32_p5 trnL-UAG-rpl32 5 AGATAAGATGTCATGCCGCC GCAATCGATTCTTACGGGGA 20 20 50.0 50.0 57.8 57.8 0.0 7.9 0.410177 127495 128086 737-737 0.500000 56.7 rpl32-ndhF_p1 rpl32-ndhF 1 TCCCCGTAAGAATCGATTGC TGTTGCCGTGGGAAAAAGTA 20 20 50.0 45.0 57.8 57.9 17.0 0.0 0.288532 128243 128953 934-934 0.500000 57.7 rpl32-ndhF_p2 rpl32-ndhF 2 TCCCCGTAAGAATCGATTGC AGGAACAGGAAGTGGAGTGA 20 20 50.0 50.0 57.8 57.9 17.0 0.0 0.337453 128243 128953 892-892 0.500000 57.3 rpl32-ndhF_p3 rpl32-ndhF 3 TCCCCGTAAGAATCGATTGC TTTGCTGCAATAGGACGTGT 20 20 50.0 45.0 57.8 58.1 17.0 16.8 0.343423 128243 128953 1353-1353 0.500000 57.3 rpl32-ndhF_p4 rpl32-ndhF 4 TCCCCGTAAGAATCGATTGC GCAATAGGACGTGTGAACCA 20 20 50.0 50.0 57.8 58.2 17.0 0.0 0.430966 128243 128953 1347-1347 0.500000 56.6 rpl32-ndhF_p5 rpl32-ndhF 5 TCCCCGTAAGAATCGATTGC TGCAATAGGACGTGTGAACC 20 20 50.0 50.0 57.8 58.2 17.0 0.0 0.430966 128243 128953 1348-1348 0.500000 56.6 ndhF_p1 ndhF 1 TGGCCCATTTTAACTTTGTGC CACCACATTCTCCATAGGGC 21 20 42.9 55.0 57.9 58.0 0.0 0.0 1.146033 128954 131197 2807-2807 0.500000 50.8 ndhF_p2 ndhF 2 TGGCCCATTTTAACTTTGTGC GCTCGGGTTATGGAAGAAGG 21 20 42.9 55.0 57.9 58.0 0.0 0.0 1.163978 128954 131197 2764-2764 0.500000 50.7 ndhF_p3 ndhF 3 TGGCCCATTTTAACTTTGTGC ATGGAAGAAGGGACCGAGAA 21 20 42.9 50.0 57.9 58.0 0.0 0.0 1.166284 128954 131197 2755-2755 0.500000 50.7 ndhF_p4 ndhF 4 TGGCCCATTTTAACTTTGTGC AGGGCCCTCTTATCTCTTCC 21 20 42.9 55.0 57.9 57.9 0.0 22.3 1.229658 128954 131197 2792-2792 0.500000 50.2 ndhF_p5 ndhF 5 TGGCCCATTTTAACTTTGTGC GGGCCCTCTTATCTCTTCCT 21 20 42.9 55.0 57.9 57.9 0.0 17.0 1.229658 128954 131197 2791-2791 0.500000 50.2