Hotspot

ycf3

LSC · 46442-48466 · 2025 bp

Back to Coulteria

Pi 0.000988

MarkerSeek score 58.20

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2025
variable_sites: 2
pi: 0.000988
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGGGGACAACAAACAATTAA	ACAATTCCTTTGTTTGTGGGA	35.0	38.1	53.2	56.2	2200 bp	1.0	40.1
ycf3_p2	TTTATTTGACTGATGGGGAC	ACAATTCCTTTGTTTGTGGGA	40.0	38.1	52.8	56.2	2213 bp	1.0	40.1
ycf3_p3	TGGGGACAACAAACAATTAAT	ACAATTCCTTTGTTTGTGGGA	33.3	38.1	53.8	56.2	2200 bp	1.0	40.1
ycf3_p4	TGGGGACAACAAACAATTAA	TGTACAATTCCTTTGTTTGTGGG	35.0	39.1	53.2	57.7	2203 bp	1.0	40.1
ycf3_p5	TGGGGACAACAAACAATTAATT	ACAATTCCTTTGTTTGTGGGA	31.8	38.1	54.7	56.2	2200 bp	1.0	40.1