Hotspot

rps16

LSC · 5485-6623 · 1139 bp

Back to Coulteria

Pi 0.000878

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1139
variable_sites: 1
pi: 0.000878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GAATAGAGCAAGAGAGCGGG	GGCCCCAAACTAGAGGAATG	55.0	55.0	57.9	57.9	1261-1266 bp	1.0	78.7
rps16_p2	ACAAGTCATCCACACCCTCT	GGCCCCAAACTAGAGGAATG	50.0	55.0	58.3	57.9	1202 bp	1.0	77.5
rps16_p3	GAATAGAGCAAGAGAGCGGG	TGGCCCCAAACTAGAGGAAT	55.0	50.0	57.9	58.3	1262-1267 bp	1.0	76.6
rps16_p4	GAATAGAGCAAGAGAGCGGG	TACTGGCCCCAAACTAGAGG	55.0	55.0	57.9	58.4	1265-1270 bp	1.0	75.8
rps16_p5	ACAAGTCATCCACACCCTCT	TGGCCCCAAACTAGAGGAAT	50.0	50.0	58.3	58.3	1203 bp	1.0	75.4