Hotspot

rpoC1

LSC · 22540-25375 · 2836 bp

Back to Coulteria

Pi 0.00106

MarkerSeek score 56.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2836
variable_sites: 3
pi: 0.00106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCGAATTCACACCGGTATCA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.7	57.9	2991 bp	1.0	76.9
rpoC1_p2	CCCGAATTCACACCGGTATC	ACGATCTTTGGCTCTGGAAC	55.0	50.0	58.4	57.9	2992 bp	1.0	75.9
rpoC1_p3	AGAAACCCGAATTCACACCG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.5	57.9	2997 bp	1.0	75.4
rpoC1_p4	CCGAATTCACACCGGTATCA	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.7	57.6	2990 bp	1.0	74.5
rpoC1_p5	CCCGAATTCACACCGGTATC	CGATCTTTGGCTCTGGAACT	55.0	50.0	58.4	57.6	2991 bp	1.0	73.6