Hotspot

cemA

LSC · 65422-66111 · 690 bp

Back to Coulteria

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TGACGAATGAGGTGAAGCTG	TATAGGAGGAATCCCCCGGA	50.0	55.0	57.9	58.5	845 bp	1.0	75.2
cemA_p2	TGACGAATGAGGTGAAGCTG	ATATAGGAGGAATCCCCCGG	50.0	55.0	57.9	57.4	846 bp	1.0	74.9
cemA_p3	GACGAATGAGGTGAAGCTGA	TATAGGAGGAATCCCCCGGA	50.0	55.0	57.6	58.5	844 bp	1.0	72.9
cemA_p4	ACGAATGAGGTGAAGCTGAG	TATAGGAGGAATCCCCCGGA	50.0	55.0	57.6	58.5	843 bp	1.0	72.9
cemA_p5	CGAATGAGGTGAAGCTGAGT	TATAGGAGGAATCCCCCGGA	50.0	55.0	57.6	58.5	842 bp	1.0	72.9