Hotspot

ccsA

SSC · 119979-120950 · 972 bp

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Pi 0.00206

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCACGGCATCTTATTTTCT	TGTCAGGCCCTTTACTCACT	45.0	50.0	56.5	58.3	1100 bp	1.0	65.8
ccsA_p2	GGCACGGCATCTTATTTTCT	TTGTCAGGCCCTTTACTCAC	45.0	50.0	56.5	57.4	1101 bp	1.0	63.5
ccsA_p3	GGCACGGCATCTTATTTTCT	GTCAGGCCCTTTACTCACTT	45.0	50.0	56.5	57.1	1099 bp	1.0	61.1
ccsA_p4	GGCACGGCATCTTATTTTCT	TTTGTCAGGCCCTTTACTCAC	45.0	47.6	56.5	58.1	1102 bp	1.0	58.8
ccsA_p5	GGCACGGCATCTTATTTTCT	GTCAGGCCCTTTACTCACTTT	45.0	47.6	56.5	57.9	1099 bp	1.0	58.8