Hotspot

accD-psaI

LSC · 63754-64109 · 356 bp

Back to Coulteria

Pi 0.00562

MarkerSeek score 56.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 2
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGCGTAATCCTTTAAAGGGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.2	58.0	503 bp	1.0	74.1
accD-psaI_p2	GCGTAATCCTTTAAAGGGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.2	58.0	502 bp	1.0	74.1
accD-psaI_p3	CGCGTAATCCTTTAAAGGGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.2	57.9	509 bp	1.0	73.5
accD-psaI_p4	GCGTAATCCTTTAAAGGGCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.2	57.9	508 bp	1.0	73.5
accD-psaI_p5	AAGGGCGTTCTTAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.8	58.0	489 bp	1.0	70.3