Hotspot

trnT-GGU-psbD

LSC · 35003-36500 · 1498 bp

Back to Coulteria

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1498
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1649 bp	1.0	78.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	55.0	50.0	58.1	58.2	1650 bp	1.0	78.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1651 bp	1.0	78.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CACAAAAACGAAACGGTCCC	50.0	50.0	58.1	58.2	1652 bp	1.0	78.0
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCACAAAAACGAAACGGTCC	50.0	50.0	58.1	58.2	1653 bp	1.0	78.0