Hotspot

rpoB-trnC-GCA

LSC · 28615-29800 · 1186 bp

Back to Coulteria

Pi 0.000861

MarkerSeek score 60.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 1
pi: 0.000861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACATTCCCGCATTTTCACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1252-1277 bp	1.0	78.8
rpoB-trnC-GCA_p2	GACATTCCCGCATTTTCACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1234-1259 bp	1.0	76.6
rpoB-trnC-GCA_p3	GACATTCCCGCATTTTCACC	GCAATTTTGTCGATCAGGCG	50.0	50.0	57.7	58.5	1276-1301 bp	1.0	75.7
rpoB-trnC-GCA_p4	TGTATCTGATTCAACCCGGG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.0	58.1	1279-1304 bp	1.0	73.0
rpoB-trnC-GCA_p5	CTGATTCAACCCGGGTATTGT	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1274-1299 bp	1.0	73.0