Hotspot

trnQ-UUG-psbK

LSC · 7363-7927 · 565 bp

Back to Coulteria

Pi 0.00177

MarkerSeek score 59.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 1
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TACTCTGGGACGGAAGGATT	GCGTAGGCTTCCGGTAATTT	50.0	50.0	57.5	58.3	732-733 bp	1.0	73.1
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	GCGTAGGCTTCCGGTAATTT	50.0	50.0	58.7	58.3	694-695 bp	1.0	72.1
trnQ-UUG-psbK_p3	ATACTCTGGGACGGAAGGAT	GCGTAGGCTTCCGGTAATTT	50.0	50.0	57.2	58.3	733-734 bp	1.0	71.3
trnQ-UUG-psbK_p4	ACTCTGGGACGGAAGGATTC	GCGTAGGCTTCCGGTAATTT	55.0	50.0	58.8	58.3	731-732 bp	1.0	71.1
trnQ-UUG-psbK_p5	TACTCTGGGACGGAAGGATT	GCTTCCGGTAATTTTGCGAA	50.0	45.0	57.5	57.4	726-727 bp	1.0	70.9