Hotspot

ndhF-rpl32

SSC · 118079-119201 · 1123 bp

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Pi 0.00901

MarkerSeek score 69.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1123
variable_sites: 37
pi: 0.00901
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGCCGAGAAAAGTAGAAGT	AAAAAGCTTTCAACGCGGTC	50.0	45.0	57.6	58.2	1277-1397 bp	1.0	81.5
ndhF-rpl32_p2	CCGCCGAGAAAAGTAGAAGT	GAAAAAGCTTTCAACGCGGT	50.0	45.0	57.6	58.2	1278-1398 bp	1.0	81.5
ndhF-rpl32_p3	CCGCCGAGAAAAGTAGAAGT	TTCAACGCGGTCCAATATCC	50.0	50.0	57.6	58.3	1269-1389 bp	1.0	80.2
ndhF-rpl32_p4	CCGCCGAGAAAAGTAGAAGT	ACGAAAAAGCTTTCAACGCG	50.0	45.0	57.6	58.5	1280-1400 bp	1.0	78.5
ndhF-rpl32_p5	GCCGCCGAGAAAAGTAGAAG	AAAAAGCTTTCAACGCGGTC	55.0	45.0	59.0	58.2	1278-1398 bp	1.0	76.4