Hotspot

rpl20-rps12

LSC · 72989-73829 · 841 bp

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Pi 0.0033

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 10
pi: 0.0033
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGCGCTATATATCCTCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	878-900 bp	1.0	80.1
rpl20-rps12_p2	TCCGCGCTATATATCCTCGT	AGGAACATGTACTCGGGTGT	50.0	50.0	58.0	58.4	877-899 bp	1.0	79.6
rpl20-rps12_p3	TCCGCGCTATATATCCTCGT	AAATGTCACCAAATCCCCCG	50.0	50.0	58.0	58.4	922-944 bp	1.0	78.7
rpl20-rps12_p4	AGTAAGTCTTGAATGCGCCC	GAGGAACATGTACTCGGGTG	50.0	55.0	58.3	57.7	940-962 bp	1.0	78.2
rpl20-rps12_p5	AGTAAGTCTTGAATGCGCCC	AGGAACATGTACTCGGGTGT	50.0	50.0	58.3	58.4	939-961 bp	1.0	77.6