Hotspot

psaJ-rpl33

LSC · 71032-71558 · 527 bp

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Pi 0.00955

MarkerSeek score 65.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 12
pi: 0.00955
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGTTGACATTCCCGTTTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	563-584 bp	1.0	82.7
psaJ-rpl33_p2	ATCAATCGCTTTTTCCCGGA	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	584-605 bp	1.0	82.5
psaJ-rpl33_p3	TCAATCGCTTTTTCCCGGAT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	583-604 bp	1.0	82.5
psaJ-rpl33_p4	CAATCGCTTTTTCCCGGATG	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	582-603 bp	1.0	82.0
psaJ-rpl33_p5	TACTCTATGGTTCGGGGCTT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	624-645 bp	1.0	81.8