Hotspot

rbcL-accD

LSC · 59357-60174 · 818 bp

Back to Couepia

Pi 0.00378

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 11
pi: 0.00378
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	AACATCGAATTGAACCACCA	50.0	40.0	58.0	55.6	872-925 bp	1.0	64.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	AACATCGAATTGAACCACCA	55.0	40.0	58.0	55.6	905-958 bp	1.0	64.2
rbcL-accD_p3	AAATGGAGTCCTGAACTGGC	AACATCGAATTGAACCACCA	50.0	40.0	57.8	55.6	896-949 bp	1.0	62.9
rbcL-accD_p4	GCTTGTGAAGTGTGGAAGGA	ACATCGAATTGAACCACCAT	50.0	40.0	58.0	55.3	871-924 bp	1.0	62.5
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCACCAT	55.0	40.0	58.0	55.3	904-957 bp	1.0	62.3