Hotspot

ndhC-trnV-UAC

LSC · 52851-54039 · 1189 bp

Back to Couepia

Pi 0.00261

MarkerSeek score 61.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 11
pi: 0.00261
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATGGGGGATAAAACTCCGGA	GCATGTTGGGTCTTTGAAGC	50.0	50.0	58.1	57.9	1329-1387 bp	1.0	83.0
ndhC-trnV-UAC_p2	CTGGACCTTTGGTAATGGGG	GCATGTTGGGTCTTTGAAGC	55.0	50.0	57.9	57.9	1343-1401 bp	1.0	82.5
ndhC-trnV-UAC_p3	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	45.0	50.0	58.2	57.9	1315-1373 bp	1.0	82.4
ndhC-trnV-UAC_p4	ATGGGGGATAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1265-1323 bp	1.0	82.1
ndhC-trnV-UAC_p5	CTGGACCTTTGGTAATGGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	1279-1337 bp	1.0	81.7