Hotspot

trnR-UCU-atpA

LSC · 9878-10159 · 282 bp

Back to Couepia

Pi 0.0158

MarkerSeek score 62.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 17
pi: 0.0158
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAAAAGCGTCCATTGTCT	ATTCGGGAACAGATGGAACG	45.0	50.0	57.7	58.0	387-434 bp	1.0	84.9
trnR-UCU-atpA_p2	TGGAAAAGCGTCCATTGTCT	CGGGAACAGATGGAACGTTT	45.0	50.0	57.7	58.5	384-431 bp	1.0	81.3
trnR-UCU-atpA_p3	TTTGGAATGGAAAAGCGTCC	ATTCGGGAACAGATGGAACG	45.0	50.0	56.9	58.0	394-441 bp	1.0	78.2
trnR-UCU-atpA_p4	TGGAAAAGCGTCCATTGTCT	AAACATTCACCGAAGAGGCG	45.0	50.0	57.7	58.8	428-475 bp	1.0	78.1
trnR-UCU-atpA_p5	TGGAAAAGCGTCCATTGTCT	GCTATTCGGGAACAGATGGA	45.0	50.0	57.7	57.1	390-437 bp	1.0	77.8