Hotspot

rpl32-trnL-UAG

SSC · 117406-118842 · 1437 bp

Back to Cotoneaster

Pi 0.00522

MarkerSeek score 48.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1437
variable_sites: 49
pi: 0.00522
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1535-1592 bp	1.0	84.2
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1536-1593 bp	1.0	84.2
rpl32-trnL-UAG_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1537-1594 bp	1.0	83.8
rpl32-trnL-UAG_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1538-1595 bp	1.0	83.8
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1545-1602 bp	1.0	83.2