Hotspot

rpl22-rps19

LSC · 87366-87426 · 61 bp

Back to Cotoneaster

Pi 0.00751

MarkerSeek score 52.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 4
pi: 0.00751
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGCCCACGAATTTGATCAAT	TCTAAATTTCCGGGGGCATG	45.0	50.0	57.2	57.9	215-249 bp	1.0	75.9
rpl22-rps19_p2	CGCCCACGAATTTGATCAAT	CTCTAAATTTCCGGGGGCAT	45.0	50.0	57.2	57.6	216-250 bp	1.0	73.5
rpl22-rps19_p3	CGCCCACGAATTTGATCAATT	TCTAAATTTCCGGGGGCATG	42.9	50.0	57.9	57.9	215-249 bp	1.0	73.5
rpl22-rps19_p4	CCGCCCACGAATTTGATCAA	TCTAAATTTCCGGGGGCATG	50.0	50.0	59.2	57.9	216-250 bp	1.0	73.2
rpl22-rps19_p5	CGCCCACGAATTTGATCAAT	ACGGATCGTATGGTAGGACA	45.0	50.0	57.2	57.6	259-293 bp	1.0	73.1