Hotspot

rpl33-rps18

LSC · 70893-71230 · 338 bp

Back to Cotoneaster

Pi 0.0217

MarkerSeek score 56.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 42
pi: 0.0217
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	389-531 bp	1.0	85.4
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	388-530 bp	1.0	83.0
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	390-532 bp	1.0	82.9
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	386-528 bp	1.0	82.4
rpl33-rps18_p5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	394-536 bp	1.0	81.9