Hotspot

petG-trnW-CCA

LSC · 69265-69388 · 124 bp

Back to Cotoneaster

Pi 0.0365

MarkerSeek score 63.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 12
pi: 0.0365
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	50.0	50.0	57.8	57.9	172-173 bp	1.0	86.7
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	50.0	50.0	57.8	57.9	173-174 bp	1.0	86.6
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	191-192 bp	1.0	86.0
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	205-206 bp	1.0	85.8
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	227-228 bp	1.0	85.3